Ex vivo HSC expansion challenges the paradigm of unidirectional human hematopoiesis
نویسندگان
چکیده
منابع مشابه
HSC Niche Biology and HSC Expansion Ex Vivo.
Hematopoietic stem cell (HSC) transplantation can restore a new functional hematopoietic system in recipients in cases where the system of the recipient is not functional or for example is leukemic. However, the number of available donor HSCs is often too low for successful transplantation. Expansion of HSCs and thus HSC self-renewal ex vivo would greatly improve transplantation therapy in the ...
متن کاملHEMATOPOIESIS Long-Term Ex Vivo Maintenance and Expansion of Transplantable Human Hematopoietic Stem Cells
We have developed a stromal-based in vitro culture system that facilitates ex vivo expansion of transplantable CD341 thy-11 cells using long-term hematopoietic reconstitution in severe combined immunodeficient-human (SCID-hu) mice as an in vivo assay for transplantable human hematopoietic stem cells (HSCs). The addition of leukemia inhibitory factor (LIF) to purified CD341 thy-11 cells on AC6.2...
متن کاملEx vivo mimicry of normal and abnormal human hematopoiesis.
Hematopoietic stem cells require a unique microenvironment in order to sustain blood cell formation; the bone marrow (BM) is a complex three-dimensional (3D) tissue wherein hematopoiesis is regulated by spatially organized cellular microenvironments termed niches. The organization of the BM niches is critical for the function or dysfunction of normal or malignant BM(5). Therefore a better under...
متن کاملEx vivo expansion of human hematopoietic stem and progenitor cells.
Despite progress in our understanding of the growth factors that support the progressive maturation of the various cell lineages of the hematopoietic system, less is known about factors that govern the self-renewal of hematopoietic stem and progenitor cells (HSPCs), and our ability to expand human HSPC numbers ex vivo remains limited. Interest in stem cell expansion has been heightened by the i...
متن کاملEx vivo expansion of human hematopoietic stem cells.
There has been great interest in the ex vivo expansion of human long-term repopulating hematopoietic stem cells (LTR-HSCs) for a variety of clinical applications such as umbilical cord blood transplantation. The glucoprotein130 signal, activated by a complex of interleukin 6 (IL-6) and soluble IL-6 receptor (IL-6/sIL-6R), acts dramatically in synergy with the c-Kit or Flk2/Flt3 signal to expand...
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ژورنال
عنوان ژورنال: Annals of the New York Academy of Sciences
سال: 2019
ISSN: 0077-8923,1749-6632
DOI: 10.1111/nyas.14133